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In mesenchymal cancer stem cells, NNMT overexpression depleted intracellular NAM and therefore enhanced the activity of PARP1, increasing the chemoradiotherapy resistance of cancer cells [48]

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RAS proteins were isolated from lysates by immunocapture on anti-RAS beads, eluted proteins were denatured (8M urea), free thiols were alkylated with IAc, and proteins were digested with trypsin prior to mass spectrometric analysis